General description

Peroxynitrite (ONOO-) is a strong oxidant generated from the interaction of nitric oxide (NO) and superoxide (O). The in vivo formation of this compound has recently been demonstrated in macrophages, neutrophils and endothelial cells. The role of ONOO- in inflammatory diseases is further supported by the detection of immunoreactive nitrotyrosine on proteins at sites of inflammation.

The BioTracker 515 Green ONOO- Dye is a fluorescent probe that detects peroxynitrite (ONOO-) in living cells. Fluorescent intensity is not increased by the existence of hydroxyl radical, singlet oxygen, hydrogen peroxide, hypochlorite, nitric oxide or superoxide.

Spectral Properties
Absorbance: 490nm
Emission: 515nm

Storage and Stability

Store BioTracker 515 Green ONOO- Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.